Energized Biomedical Sciences Reseacher

Administrative Assistant/Office Manager

2013-present

OnTrac Investigative Group; Nashville, TN

• Perform Investigative background checks and comprehensive reports for clients
• Performs secretarial duties, data entry, receives and screens office calls and correspondence, responds to routine telephone inquiries; forwards calls to appropriate party.
• Organizes, secures and maintains confidential and sensitive  materials

• Design, update and manage company website
• Design and make employee badges
• Confirm, submit and process payroll for company employee’s
• Order supplies and run necessary errands for upper management
• Generate and submit invoices

Research Assistant II – Neonatology

2012-2013

Vanderbilt University Medical Center; Nashville, TN

• Independently performed & recorded complex standardized and non-standardized scientific protocols and lab procedures
• Fixative processed, microtome/cryostat sectioned, stained  and interpreted tissues for morphology and/or immune-histological analysis
• Conducted pulmonary surgeries on model neonates and adult animals

• Managed mouse colony and performed DNA genotyping for multiple genetic alleles
• Experience working with mouse models of infections
• Troubleshooting/repairing laboratory equipment
• Conducted fluorescence and confocal microscopy studies

Graduate Researcher - Cardiovascular Pharmacology

2008-2012

Vanderbilt University Medical Center; Nashville, TN.

• Performed validation Quantitative PCR for publications
• Expressed technical information clearly, both orally and in writing
• Maintained, amplified and validated various adenovirus mutant strains under ABSL2+ conditions
• Conducted long term mouse blood pressure, echocardiography, weight and morphology study

• Supervised, trained and mentored students in embryonic micro dissection and basic laboratory safety/maintenance
• Designed and implemented siRNA constructs
• Developed  a cell culture system to validate ex vivo studies
• Assisted PI in modifying procedures and techniques

Post- Baccalaureate Researcher - Veterinary Immunology

2007-2008

Virginia-Maryland Regional College of Veterinary Medicine; Blacksburg, VA

• Validated Protein purification and expression
• Contributed to development of a porcine Immunocontraceptive Vaccine
• Implemented restriction mapping and Immunoblotting in vaccine design
• Extensive bacterial culture system experience

Research Intern - Toxicology and Pharmacology

Summer 2006

Boonshoft School of Medicine, Wright State University; Dayton, OH

• Learned and utilized In Situ Hybridization techniques and radio-immunoassay
• Mastered Cryostat sectioning
• Presented findings at national conference and won 1st place in physiology category

Undergraduate Researcher - Plant Biochemistry

2002-2006

Virginia Polytechnic Institute and State University; Blacksburg, VA

• Solidified DNA/RNA isolation skills
• Effectively learned and used PCR
• Implemented Plasmid application/transfection into model
• Trained in Confocal microscopy
• Learned Thin-layer chromatography