GetHiredToday

Senior Research Associate. Department of Chemistry, Institute for Lasers Photoni

Posted on: 2011-07-06

Dr. Adela C. Bonoiu

 

 

EDUCATION

MSc l Biochemistry and Molecular Biology, University of Bucharest, Dept of Biochemistry, Romania(2003)
PhD Biochemistry  University of Biology, University of Bucharest, Dept of Biochemistry, Romania (2006)  

 

CAREER GOALS

Excellent Nanotechnology Biomedical and Molecular Medicine Research Scientist seeks for a new permanent career path. Hands-on experience in biomedical science, molecular medicine, space life sciences and biotechnology. Aim to work in compatible Biotechnology and/or Pharmaceutical Industry R & D labs as well as in Science Labs.

 

LIFE SKILLS

Excellent problem solving and negotiation skills. Works well in multicultural environments;

 Optimistic and positive view to life and future and mentoring attitude towards colleagues;
 Encouraging team player – want to seek opportunities to team’s best and advancements;
 My Leadership model is based on my own example – it is doing more, talking less – or walk your talk;
 Multitasker and achiever, can make things to happen, likes to work in multiple projects;

 

WORK EXPERIENCE

04/2010 - Present Senior Research Associate. Department of Chemistry, Institute for Lasers Photonics and Biophotonics, SUNY Buffalo, NY

  • Preparation  scientific presentation and proposals
  • Investigate the effect of  gene silencing on chronic pain behavior
  • Investigate the effect of  ssRNA nanoplexes on activation of the innate immune response.

 

05/2006 - 04/2010 Postdoctoral Fellow . Department of Chemistry, Institute for Lasers Photonics and Biophotonics, SUNY Buffalo, NY

  • Development of nanoformulation for gene silencing in rat hippocampus.
  • Identification and selection of nanoparticles as gene carrier for gene silencing or augmentation in vitro and in vivo.
  • Investigate the effect of DARPP-32 silencing in improving the addictive behavior

 

10/2005-01/2006  Research Scientist, Department of Biochemistry, University at Bucharest

  • Investigation the effect of UVA and UVB on the morphology of skin cells;
ADDITIONAL TRAINING
10/2002-02/2003 University of Limoges, Limoges France Master Thesis Experimental part;
07/2004 Summer School Iassy, Romania Molecular Biology;
07/2009 Centre for Diagnostic and Control Atlanta, Gene delivery using nanopartcles for viral therapy;

 

SPECIAL COMPETENCIES AND INTERESTS

Nanotechnology with medical application: gene transfection using nanoparticles formulation, methods for testing the gene transfection in vivo and in vitro,  cell toxicity after nanoparticles treatment.

Cell culture: primary cells line (fibroblast, kerationocyte, melanocyte, neurons), brain blood barriers constructs, BMVEC, COS 1,7, HEK, HeLa cells.

Molecule biology: DNA recombination, plasmid/retroviral plasmid construction, subcloning, sequencing of PCR, stable/transient transfection by Lipofectamine, FuGene 6, retrovirus, Nucleofector and Electroporation, siRNA, PCR, RT-PCR, situ hybridization, DNA and RNA isolation.

Cell biology: Cell lines/primary cell culture, cell growth viability curve, cell apoptosis and differentiation assays, immunofluorescent double/triple staining, cell sorting by FACS, gel zimography;

 Microscopy: Light microscopy, fluorescent/laser confocal microscopy, Dark Field Microscopy, Raman and CARS microscopy

Virology:  Influenza Virus plaque assay

Immunology: Culture and transfection, luciferase assay, ChIP assay, ELISA, Western blotting, immunohistochemistry, flow cytometry

 

LANGUAGE SKILLS

Romanian, mother language (excellent)
English, spoken and written (excellent)

French, spoken and written (good)
Russian, basic

 

PUBLICATIONS (Display 4 from a list of 14 publications and one  US patent application)

 

Adela C. Bonoiu, Earl J. Bergey, Hong Ding, Rui Hu, Rajiv Kumar, Ken-Tye Yong, Paras N. Prasad, Supriya Mahajan, Kelly E. Picchione, Arin Bhattacharjee, Tracey A. Ignatowski Gold Nanorod-siRNA Induces Efficient In Vivo Gene Silencing in the Rat Hippocampus. Accepted Future Nanomedicine 2010 (On-line June 2011).

 

Chakravarthy, K.V., Bonoiu, A.C., Davis, W.G., Rajan, P., Ding, H, Hu., Rui, Bowzard, J.B., Bergey, E.J., Katz, M.J., Knight P.R., Sambhara, S., & Prasad, P.N. (2010). Gold nanorod delivery of an ssRNA immune activator inhibits pandemic H1N1 influenza viral replication. PNAS 107(22), 10172-10177;

 

Bonoiu, A.C., Mahajan, S.D., Ding, H., Roy, I., Yong, K.Y., Kumar, K., Hu, R., Bergey, E.J., Schwartz, S.A., & Prasad, P.N., (2009). Nanotechnology approach for drug addiction therapy: gene silencing using delivery of gold nanorod-siRNA nanoplex in dopaminergic neurons, 106(14):5546-5550.

 

Bonoiu, A.C., Mahajan, S.D., Ye, L., Kumar, R., Ding, H., Yong, K.Y., Roy, I., Aalinkeel, R., Nair, B., Reynolds, J.L., Sykes, D.E., Imperiale, M.A., Bergey, E.J., Schwartz, S.A.,  & Prasad, P.N., (2009). MMP-9 gene silencing by a quantum dot-siRNA nanoplex delivery to maintain the integrity of the blood-brain barrier.  Brain Res, 1282, 142-155.

 

 

REVIEWER FOR JOURNALS

Nanoscale , Molecular Biosystem ,Analyst , Soft Matter , Journal of Materials Chemistry, Integrative Biology

 

MEMBERSHIPS

American Chemical Society, American Pain Society