Mtech ANNA univ, Chennai admitted through JNU

Posted on: 2010-01-27

 

 

PROJECTS UNDERTAKEN

 

 

• To study the effect of active extracts of medicinal plant CBT-023 for cell cycle analysis followed by apoptosis in A549 and Differentiation therapy in  HL-60 cell line in-vitro.                                          

(SPIC BIOPROCESS Lab Taramani  Chennai (Anna University) 

Guide: Dr. B.S. Lakshmi                                                                    (M.Tech Project)   

                          Lecturer (Center of Biotechnology)Anna University

               Team Size: 2                                                                          

(Abstract): Selection of Medicinal plant followed by sequential extraction with solvents ranging from non polar to polar was done and extracts were resolved using Thin layer chromatography to check its major components. Then, Anti-cancer activity of methanol and dichloromethane extract of medicinal plant was tested in HL-60 and A549 cells invitro. Two hallmarks of cancer therapy viz differentiation and cell cycle arrest were considered as the best way to eliminate cancer cells. Cell viability and proliferation was checked by MTT assay and 3[H] thymidine incorporation assay respectively. Differentiation in HL-60 Cell lines was assayed by Geimsa staining. Cell cycle arrest in HL60 and A549 was determined by flow cytometry and confirmed by analyzing the expression of CDK 2, 4, 6, Cyclin E, D1, D3 and cell cycle inhibitor P16 and P27 by Western Blotting. Both differentiation and cell cycle arrest leads to Apoptosis which was analysed by DNA fragmentation and confirmed by protein expression of PARP and gene level expression of Caspase 3and 8 using RT PCR.

 

 

• Comparative study on Epitope prediction in Salmonella typhi CT18 

(ACS-Bioinformatics, Biotech Park,Lucknow)                                                  

Guide:   Mr. Nitin Chitranshi                                                                               (B.E. project)

              Team Size -1

 

 

 

 

 

INDUSTRIAL TRAINING AND RESEARCH EXPOSURE

 

 

 

• Mohan Meakin Limited

       (Lucknow distillery, Daliganj, Lucknow, UP)                                                            (1 month)

 

Mohan Meakin, a large group of companies incorporated in 1855, specializes in the   manufacture of brewers, distillers, liquor, mineral water, glass bottles, beer. I worked  on  Fermentation techniques of industrial alcohol and beer production, including Effluent Treatment,  Water Treatment and Bottling..

 

            

• Cream Bell 

(Universal dairy Products Pvt. Limited, Agra)                                                        (1 month)

Guide:   Mr. Sanjeev Chauhan and Mr. I.P.Singh                                               

• Parag Dairy 

 (Kanpur,UP)                                                                                                   (15 days)                    

.

      Learnt manufacturing, processing,  quality control of milk and milk    products.

      

 

• National Jalma Institute for Leprosy and other Mycobacterial diseases

(Tajgang,Agra-U.P.)                                                                                                      (15days) 

Guide:  Dr. V.M. Katoch

 

Institute, established in 1976, focuses mainly on research on Leprosy, Tuberculosis and HIV infection. During my training I got a hands-on experience on molecular biology and bio-analytical techniques like ELISA, PCR, westen bottling.

 

 

• Paliwal Institute of Medical Sciences

(Kanpur, UP)                                                                                                                             (15 days)  

 

       Worked on clinical microbiology and sterilization techniques.

 

 

 

 

 

 

 

 

 

 

SEMINARS  AND CONFERENCE UNDERTAKEN

 

  

 

• Presented a poster in the 5th “World Congress of Cellular and Molecular Biology” jointly organized by the School of Biotechnology, Devi Ahilya University, Indore, India and world Society of Cellular and Molecular Biology, France.

• Indo Russian Seminar on Biotechnology held at Anna University Chennai.
• Biotechnology: UK and India Perspectives 2008 organized jointly by British Council and Anna University, Chennai 
• Biotechnology: UK and India Perspectives 2009 organized jointly by British Council and Anna University, Chennai. 
• Emerging Trends in Stemcell &Tissue Engineering Research held by Frountier Tissueline. 

 

 

ACHIEVEMENTS

 

 

 

• Recipient of Merit scholarship from Department of Biotechnology, Govt. of India for M.Tech Biotechnology at Anna University. 

• Qualified GATE with 93.21% or 400 score.
• Qualified All India JNU Entrance test for M.Tech Biotechnology.
• University topper during B.E.

 

EDUCATIONAL QUALIFICATIONS

 

S.No	Institution	Board/University	Year	Course	GPA/Percentage
1	Anna University, Chennai	Anna University, Chennai	2008-10	M.Tech (Biotechnology)	CGPA – 9.2*
2	Dr. B. R. Ambedkar University, Agra	Dr. B. R. Ambedkar University, Agra	2004-08	B.E. (Biotechnology)	82.35%
3	Dr. Virendra Swarup Education Centre, Kanpur	ISC	2002-03	Intermediate	77%(aggregate)
4	Dr. Virendra Swarup Education Centre, Kanpur	ICSE	2000-01	High School	79.5%(aggregate)

*until 2th semester

AREAS OF INTEREST

 

 

• Cell and Molecular biology techniques
• Bioanalytical techniques
• Bioprocess Engineering techniques.

 

SKILLS

 

 

• Computer Skills : C, C++, UNIX, PERL, Microsoft Office.
• Comprehensive problem solving skills and excellent written communication skills

 

 

EXTRA-CURRICULAR ACTIVITIES

 

 

• Participated in Bio Expo conducted by the Biotechnology Society of I.E.T Khandari Agra.
• Participated in “SCOPE” quiz competition held at I.E.T. Khandari, Agra.
• Volunteer in science festival organized by Science city, Department of Higher   Education,   Govt. of Tamil Nadu.
• Got second prize in NGSC singing competition in Kanpur.