scientist III Resume
Larissa V. Ponomareva Tabor Ct, Florence, KY
• Cellular • Home
Summary of Qualifications
· Sixteen years scientific research experience and year of teaching undergraduate and graduate courses in Biochemistry, Immunochemistry, Biomembranes, Ecology
· Experience includes years biochemistry research, years molecular biology research, years cell biology, years physiology research, years electrophysiology research, and years managerial and custom services expertise
· Authored scientific research publications in peer reviewed journals, as first author and as second author
· Independent research projects include cloning of fish cytochromes P-A and A genes, studies of fish P- transcriptional regulation in comparison to murine P- promotor, development of transgenic zebra fish with GPF gene indicator and transgenic mice with degradable GFPd gene and period and fos promotors, studies of retinal glutamate receptors nitric oxide modulation and AMPA glutamate receptors cloning and expression, studies of cell signaling of chemokine mim- and its role in osteoblast cell differentiation and mineralization, and effect of proteasome inhibitors on NFkB signaling and efficacy of radiation
· Managed the successful completion of over custom research assignments and projects on the contract with US EPA
¨ Managed the launch of the Research Core Facilities at the US EPA NERL; developed training and business strategies plan for Core Research Facilities on the contract with US EPA
¨ Developed new polymorphic genetic markers and indicators for environmental exposure
¨ Implemented efficiencies that improved throughput of custom services while reducing labor, materials, and standard cost of product by %
¨ Edited scientific research articles for laboratory colleagues
¨ Generated the transgenic mice with degradable GFPd and c-fos and period- promotors for circadian clock research project
¨ Discovered that nitric oxide and cGMP modulate retinal glutamate receptors
¨ Cloned and sequenced two ionotropic glutamate receptors and two cytochrome P- genes
¨ Discovered the role of osteoclast-secreted chemokine Mim- in osteobasts differentiation and bone matrix mineralization
¨ Discovered that proteasome inhibition could enhanced radiation sensitivity in prostate cancer cells but does not have this effect in normal cells and this could be a potential way to improve efficacy of radiation in prostate cancer patients
Professional Experience
Studying the molecular mechanisms of pain regulation in pancreatic cancer and arthritis animal models
Directed - people in the daily process of custom sequencing and genotyping for the US EPA researchers and ensured effective follow-through on all assigned tasks as well as strategic delegation of tasks to qualified individuals. The group ran from to sequencing or genotyping samples per day, my responsibilities included troubleshooting, customer interface, data analysis and data presentation. One of my main contributions was process improvement and experimental design of custom research projects, developing Work Plans, SOPs and quality assurance reports.
Acquired knowledge in biological effects of proteasome inhibitor MG- on NFkB regulation in prostate cancer animal model.
Research Associate (Department of Internal Medicine)
Studied cell signaling of chemokine mim- and its role in osteoblast cell differentiation and bone mineralization
Postdoctoral Research Fellow/Research Associate (Department of Physiology)
Carried out research on function, modulation and expresiion of ionotropic glutamate receptors from fish retina, directed project on circadian clock study, generated transgenic GFP mice.
Postdoctoral Research Fellow (Department of Environmental Health)
Cloned, sequenced, and studied transcriptional regulation for trout cyt. P-A and A genes and participated in developing transgenic GFP-zebrafish-biomonitor (environmental “sentinel” for aquatic pollution)
Assistant Professor (Department of Physico-Chemical Biology)
Taught biochemistry, immunochemistry, ecology and biomembranes courses for undergraduate and graduate students
Senior Scientist (Institute of Biology)
Conducted research on effect of environmental pollution on induction of cytochrom P- isoforms from freshwater fish species including endemics from Lake Baikal
Ph.D. student (Department of Biophysics)
Irkutsk State University, Irkutsk, USSR / to /
Research Fellow (Department of Physico-Chemical Biology)
Education
Moscow State University, Moscow, Russia
Irkutsk State University, Irkutsk, USSR
Skills
• Teaching: Biochemistry, immunochemistry, biomembranes, ecology
• Management: Responsible for production of high-throughput contract sequencing and genotyping core facility. Duties included managing high-throughput robotic DNA and RNA extraction/purification, DNA genotyping and sequencing, non-isotopic library screening, large-scale gene-specific PCR amplification for genotyping and polymorphism studies, assay development and sequence data analysis, RT-QPCR analysis, preparation of Lab Work Plans, Quality Management Plans, SOPs, monthly QA/QC reports, review of all data and staff reports, managing the laboratory supply budget and supervision of a staff of scientists (PhD, MS & graduate students) and technicians (BS).
• Molecular biology expertise in: Gene Chip Microarrays, cDNA and genomic libraries construction and screening, DNA cloning, DNA extraction: minipreps, maxipreps (manual and BioRobot workstations), CsCl DNA purification; Southern, primers design, site-directed mutagenesis, footprinting, manual and high through-put automatic sequencing, SSCP, SNP, genotyping (microsatellite), Genome Walk, RNA purification, Northern, RNase mapping, in vitro transcription, RT-PCR, single cells RT-PCR, in situ hybridization, RACE, primer extension, RT-QPCR (TaqMan, SYBR Green), differential display
• Biochemistry expertise in: EMSA, SDS PAGE, D gels, Western Blotting, HLPC, ELISA, IHC, protein extraction, dialyze and protein purification, immunoprecipitation, binding assays, receptor/ligand interaction, protein expression in bacterial and mammalian cell lines, labeling/conjunction, immunocytochemistry, enzyme assays and enzyme kinetics, IEC, TLC, mass-spectrometry, GLC, flow cytometry and cell sorting (FACS), spectrophotometry, spectrofluorimetry
• Computers and programming: Word processing, spreadsheet, PowerPoint and computer data analysis (BLAST, SigmaPlot, Excel, Axon Workbench, Clampex-, AtlasImage., etc); large-scale genomic analysis
• Tissue culture expertise in: Transient transfections of HEK and NA cell lines (calcium-phosphate method, Lipofectamin, Fugen, gene gun, electroporation), stable retroviral (Clontech) cell transfection, immunocytochemistry, neuronal and hepatocyte primary culture, growth of retinal cells, fibroblasts, culture MG-, human stromal cells, CC, cancer cell lines, clonogenic survival assay (+/-presence of drug, +/- radiation)
• Electrophysiology expertise in: Whole cell patch-clamp recording from retinal horizontal cells, HEK cells, recording data acquisition analysis with Clampex
• Imaging: EGFP imaging analysis, fluorescent calcium imaging analysis with fura-
• Microscopy: light-inverted microscope, epifluorescence, confocal, electron-microscopy
American Association for the Advancement of Science
Association for Research in Vision and Ophthalmology
Society for Neuroscience
Russian Biochemical Society
Managers School Training and Negotiation Skills Training completed at Xavier Consulting Group, Xavier University, Cincinnati,OH USA.
International Biotechnology Center; Biomonitoring, Environmental Health and Ecology Training Program, Moscow State University, Completed with Certification of Advanced Training
Fight for Sight Research Fellowship Award, Prevent Blindness America, ARVO, USA
Award for best poster presentation at Society of Neuroscience Conference, University of Kentucky, Lexington, KY, USA
COMECON Fellowship, Biotechnology and Ecology Training Program, Moscow
Moscow State University Fellowship, Moscow
Award of Excellence for M.S.D. Dissertation Research, USSR Competition of Graduate Student Dissertation Research, Moscow
Lenin Fellowships, Irkutsk State University, Irkutsk
Novosibirsk State University Fellowship, Novosibirsk
Gold Medal for graduation with honors, High School, Irkutsk
Furnished upon request
