Research Assistant

Posted on: 2007-12-06

Gladis Thomas 237 South 8th Street, Newark NJ 07103 215-307-6276 [email protected] SUMMARY OF QUALIFICATIONS Extensive research experience involving invertebrate and vertebrate animal models. Strong analytical and planning skills, combined with the ability to coordinate the efforts of many to meet organizational goals. Productive and efficient work habits without supervision. Self-motivator with high energy. EMPLOYMENT HISTORY & TECHNICAL SKILLS Research Assistant June, 2001-2006 Yale University Intra- and extra-cellular electrophysiological recordings using single- and two-electrode voltage clamp methods. Vibratome sectioning and immunostaining of rat and primate brains for light and electron microscopic analyses. Preparation of brain tissue for electron microscopic examination, which includes fixation, embedding, and cutting ultrathin sections. Electron microscopic unbiased stereological calculation of hippocampal CA1 pyramidal cell apical dendritic spine synapses, following different gonadal hormonal treatments. Treatment with various gonadal hormones of gonadectomized animals, involving subcutaneous and intramuscular injection of gonadal hormones and receptor antagonists following general anesthesia and gonadectomy of male and female rats. Tissue culture involving microdissection of embryonic brain to isolate and culture hippocampal neurons and cortical glial cells. Immunocytochemistry involving the use of antibodies specific for synaptic components to examine synapse formation in cultured hippocampal neurons. EDUCATION BS, Biochemistry 2001 Temple University, Philadelphia, PA Major: 2.8 GPA MS, Cellular and Molecular Biology 2006 University of New Haven, West Haven, CT Major: 3.5 GPA MS, Biomedical Science 2008 University of Medicine and Dentistry in New Jersey, Newark, NJ Major: 3.3 GPA THESIS PROJECT: Co-regulation of voltage-dependent ionic conductances and mRNA levels in neurons of a central pattern generating network: Effect of neuromodulators REFERENCES FURNISHED UPON REQUEST