Post doctoral fellow

Posted on: 2007-09-29

Angelique BIANCOTTO 15931 Indian Hills terr Derwood MD 20855 Professional address LCMB/NICHD/NIH 10, center drive Bldg 10 room 9D51 Bethesda MD 20892 Tel 1 301 496 7087 Fax 1 301 480 0857 Email [email protected] UNIVERSITY EDUCATION • 2003: Ph. D Specialty Immunology: “HIV-1 persistence in AIDS” University Aix-Marseille II, in the INSERM U372 in Marseille Luminy under supervision of Dr. Ivan Hirsch • 2000: M.S. Immunology (French Diplome d’Etudes Approfondies en Immunologie) (September 1999 to September 2000), University of Aix-Marseille II, in the INSERM U372 in Marseille Luminy under supervision of Dr. Ivan Hirsch • 1995-1999: B. S. Biochemistry and molecular biology (French Maitrise de Biochimie mention biologie moleculaire), Faculte des Sciences de Luminy, Universite Aix-Marseille II. Major in Immunology. RESEARCH EXPERIENCE September 2003 - present, Post-doctoral fellow: Study of coinfection with HIV-1 and different pathogens in an ex vivo model. Study of immune dysregulation upon HIV-1 infection. LCMB, NIH. Bethesda February 2005 - March 2005, Post-doctoral fellow: Preparation of a substractive library from cDNA of non-infectious HIV infected tonsils histocultures. St George’s hospital medical school London. July 2000 - July 2003, Ph.D. student: Characterization of latently infected T lymphocytes in an in vitro model developed in our laboratory. Analysis of the phenotype of resting memory T CD4+ cells in an ex vivo model: tonsils tissue. Utilization of HIV derived vector. INSERM U372-Marseilles August 99- July 00, Graduate student: Characterization of replication of different HIV-1 derived virus in T CD4+ cells. INSERM U372-Marseilles December 99- January 00, Graduate student: Characterization of thymic cells expressing Vanin-1. TECHNICAL EXPERTISE Cell Biology Cell cultures: Maintenance of human cell lines (adherent and in suspension), primary cultures of PBMC, cellular cloning. Cell separation with magnetics beads. Flow cytometry FACS (Calibur, Quanto, LSR II). Cell separation (Aria), Multiplex bead array Development and validation of Luminex based (Luminex, Biorad) Retrovirology Viral stocks preparation, infection, transfection, titration, RT activity, ELISA p24. Molecular Biology DNA analysis: cloning, sequencing, sequence analysis, RT-PCR, semi-quantitative RT-PCR, Real time PCR, Southern blots, Northern blots. Substractive library of cDNA. Immunochemical analysis Nuclear and cytoplasmic's proteins separation, western blots. Immunohistochemistry Mouse dissection. Thymus extraction. T lymphocytes isolation. Tonsils, lymph nodes, rectal biopsies, cervix dissection, 3D histocultures. ORIGINAL CONTRIBUTIONS IN PEER REVIEWD JOURNALS 1. Naspetti, M., Martin, F., Biancotto, A., Malergue, F., Mansuelle, P., Galland, F., and Naquet, P. 2000. A novel anti-Ep-CAM antibody to analyze the organization of thymic medulla in autoimmunity. Curr Top Microbiol Immunol 251:109-117. 2. Gondois-Rey, F., Biancotto, A., Pion, M., Chenine, A.L., Gluschankof, P., Horejsi, V., Tamalet, C., Vigne, R., and Hirsch, I. 2001. Production of HIV-1 by resting memory T lymphocytes. Aids 15:1931-1940. 3. Gondois-Rey, F., Grivel, J.C., Biancotto, A., Pion, M., Vigne, R., Margolis, L.B., and Hirsch, I. 2002. Segregation of R5 and X4 HIV-1 variants to memory T cell subsets differentially expressing CD62L in ex vivo infected human lymphoid tissue. Aids 16:1245-1249. 4. Grivel, J.C., Biancotto, A., Ito, Y., Lima, R.G., and Margolis, L.B. 2003. Bystander CD4+ T lymphocytes survive in HIV-infected human lymphoid tissue. AIDS Res Hum Retroviruses 19:211-216. 5. Pion, M., Jordan, A., Biancotto, A., Dequiedt, F., Gondois-Rey, F., Rondeau, S., Vigne, R., Hejnar, J., Verdin, E., and Hirsch, I. 2003. Transcriptional suppression of in vitro-integrated human immunodeficiency virus type 1 does not correlate with proviral DNA methylation. J Virol 77:4025-4032. 6. Biancotto, A., Grivel, J.C., Gondois-Rey, F., Bettendroffer, L., Vigne, R., Brown, S., Margolis, L.B., and Hirsch, I. 2004. Dual role of prostratin in inhibition of infection and reactivation of human immunodeficiency virus from latency in primary blood lymphocytes and lymphoid tissue. J Virol 78:10507-10515. 7. Gondois-Rey, F., Biancotto, A., Fernandez, M.A., Bettendroffer, L., Blazkova, J., Trejbalova, K., Pion, M., and Hirsch, I. 2006. R5 variants of human immunodeficiency virus type 1 preferentially infect CD62L- CD4+ T cells and are potentially resistant to nucleoside reverse transcriptase inhibitors. J Virol 80:854-865. 8. Lisco, A., Grivel, J.C., Biancotto, A., Vanpouille, C., Origgi, F., Malnati, M.S., Schols, D., Lusso, P., and Margolis, L.B. 2006. Viral Interactions in Human Lymphoid Tissue: HHV-7 Suppresses the Replication of CCR5-tropic HIV-1 via CD4 Modulation. J Virol. 9. Biancotto, A., Grivel, J.C., Iglehart, S.J., Vanpouille, C., Lisco, A., Sieg, S.F., Debernardo, R., Garate, K., Rodriguez, B., Margolis, L.B., et al. 2007. Abnormal activation and cytokine spectra in lymph nodes of persons chronically infected with HIV-1. Blood. 10. Grivel, J.C., Elliott, J., Lisco, A., Biancotto, A., Condack, C., Shattock, R.J., McGowan, I., Margolis, L., and Anton, P. 2007. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1. Aids 21:1263-1272. 11. Lusso, P., Crowley, R.W., Malnati, M.S., Di Serio, C., Ponzoni, M., Biancotto, A., Markham, P.D., and Gallo, R.C. 2007. Human herpesvirus 6A accelerates AIDS progression in macaques. Proc Natl Acad Sci U S A 104:5067-5072. 12. Vanpouille, C., Biancotto, A., Lisco, A., and Brichacek. 2007. Interactions between HIV-1 and vaccinia virus in human lymphoid tissue 
ex vivo. (J. Virol, in press) 13. Biancotto A., Iglehart SJ., Vanpouille C., Condack C.E., Lisco A., Ruecker E., Hirsch I., Margolis L. and Grivel JC. HIV-1 production and depletion of activated and non-activated cells in human lymphoid tissue ex vivo (Blood in press) ARTICLES IN REVISION OR PREPARATION 1. Biancotto A., Iglehart SJ., Lisco A., Vanpouille C., Grivel JC., Lurain N., Reichelderfer P. and Margolis L. Upregulation of Human cytomegalovirus by HIV-1 in human lymphoid tissue ex vivo. (submitted for publication to AIDS research and human retroviruses) 2. Čokić V.P., Bhattacharya B., Smith R.D., Biancotto A., Noguchi C.T., Puri R.K., and Schechter A.N.. Gene expression profiling in fetal and adult definitive erythropoiesis. (submitted for publication to BMC genomics) 3. Biancotto A., Grivel JC., Lisco A., Vanpouille C., Markham P., Gallo R., Margolis LB., and Lusso P. Evolution of SIV toward RANTES Resistance in HHV-6A-Coinfected Macaques Rapidly Progressing to AIDS (submitted for publication Nature medicine) 4. Biancotto A., Lisco A., Vanpouille C., Margolis LB., and Grivel JC. Activation and Memory phenotype in lymphoid tissue: a model for immune response. (in preparation) INVITED PARTICIPATIONS AND LECTURES Invited speaker, First annual Think Tank HIV meeting, NIH, Presentation title: “Immune dysregulation upon HIV infection in human lymphoid tissue” Bethesda. Maryland, September 14, 2007 Invited participant, CFAR meeting, Center for AIDS Research, Case Western Reserve University, Cleveland. Ohio, June 7-9, 2007 Invited participant, CLIC meeting, Center for AIDS Research, Case Western Reserve University, Cleveland. Ohio, July 15-16, 2006 Invited speaker, ISHEID meeting. Presentation title: “HHV-6 induced evolution of SIV: Guilt by association” Toulon, FRANCE, June 19-21, 2006 Invited participant, Center For Aids Research Meeting, Case Western Reserve University, Cleveland. Ohio, June 1-4, 2006 Invited speaker, Ninth Annual HIV Drug Resistance Program Think Tank. Presentation title: “In vivo HHV-6 induced evolution of SIV” Frederick, Maryland, April 11, 2006 Invited speaker, Cleveland Immunology Consortium (CLIC) meeting, Center for AIDS Research, Case Western Reserve University. Presentation title: “Abnormal cytokine profile in HIV infected lymph nodes” Cleveland. Ohio, Nov 20-21, 2005 Invited speaker, International AIDS Society meeting. Presentation title: “Dual Role of Prostratin in HIV infection” Paris, FRANCE, July 16-19, 2003 FELLOWSHIPS AND AWARDS - 2000-2003: Fellowship from the Ministère de la Recherche et Technologie. - 2003-present: Fogarty International Center: Visiting Fellow Fellowship. - 2007-Bill and Melinda Gates Foundation scholarship (keystones symposia) REFERENCES Dr Jean Charles Grivel NASA NIH Center for 3 Dimension Tissue Culture LCMB/NICHD/NIH Bldg. 10, Room 9D58 10 Center Dr. Bethesda, MD 20892 Tel. 301-496-1298 Fax: 301-480-0857 Email: [email protected] Dr Ivan Hirsch Institut de Recherche sur le Cancer de Marseille, UMR 599 INSERM Institut Paoli-Calmettes IFR 137, Institut de Cancérologie et d'Immunologie de Marseille, Université de la Méditerranée 27, boulevard Lei Roure 13009 MARSEILLE (France) Tel. +33-491-758-415 Fax. +33-491-260-364 Email: [email protected] Dr Leonid Margolis NASA NIH Center for 3 Dimension Tissue Culture LCMB/NICHD/NIH Bldg. 10, Room 9D58 10 Center Dr. Bethesda, MD 20892 Tel. 301-594-2476 Fax: 301-480-0857 Email: [email protected]