postdoctoral research associate

Posted on: 2007-03-23

CURRICULUM VITAE Name: Barbara Mariola Detweiler Maiden name: Barbara Mariola Kukielczak Mailing Address:5301 51st St Apt A13, Lubbock, TX 79414 Phone: 806-797-0766 Email: [email protected] Skills, Knowledge and Abilities: - Extensive knowledge of molecular biology, cell biology, biochemical biophysical and histological assay techniques as well as bioassay conception and implementation. Particular listing: Cell culture:monolayers - immortalized and primary cultures (HaCaT, NHEK keratinocytes, Human Fibroblasts, Retina Pigment Epithelial cells, Caco-2 cells), suspensions (Bomirski Hamster Melanoma, B16, L1210, RAW264.7, NR8383). Cell viability tests: trypan blue, LPO, MTS.Light and fluorescence microscopy, digitization and computer analysis of cell and tissue images (Kinetic, Matrox Inspector) Histological procedures: preparation of sample from in vivo through fixation and slicing (paraffin and frozen sections) to staining for light microscopy (eosine/hematoxyline), and electron microscopy (Osmium tetroxide OsO4), Myelin Basic Protein (MBP) Immunostaining, Pi-GSTstaining, TUNNEL staining, Oil Red O staining; Biochemical/biophysical assays: PCR, genotyping, DNA damage assessment by comet assay (SCGE) also used for epidemiology studies, protein content (Lowry, BCA) vs. oxidized protein content (DNPH), nitrite assay (Griess reaction), lipid peroxidation TBARS, LPO. - Applied learned techniques to study exogenous and endogenous DNA damage as it applies to photochemistry /photobiology and their impact on cancer. For example, mastery of Comet assay technique for DNA damage (1997-2003) as well as established new model for eye melanoma (1995). In addition, developed microsurgical technique for ophthalmological applications (1995). - Developed new technique for formulation of Water/Oil/Water multiple emulsion (1996). - Awarded three grants from the Polish National Grants Committee for Science (1995, Jagiellonian University, Krakow), NATO and EU (2001, Institute of Nuclear Physics, Krakow). Managed and organized cooperation between several institutions (1990-1995,1996). - Broad knowledge of computer software, including the most commercially available applications for Microsoft and - Apple Macintosh. - Analytical and clear in verbal and written presentations - Thoughtful, detail oriented, and well organized- Dependable with strong work ethic - Willing to relocate Education: 1990-1995: Ph.D. (Biophysics) Jagiellonian University, Krakow, Poland 1984 -1989: Master of Science (Molecular Biology) Jagiellonian University, Krakow, Poland 1985-1989: (Philosophy) Academy of Fine Arts in Krakow, Poland 1980-1984: Secondary School No 1 (Humanities and Art), Rabka, Poland 1973-1979: Level 1 Diploma (Piano and Flute) Rabka Music School, Poland 1972-1980: Primary School No 1 in Rabka, Poland Professional Employment: 2004 - 2005: Postdoctoral Research Associate, Department of Pathology and Laboratory Medicine, University of North Carolina Medical School, Chapel Hill, NC USA 2003 - 2003: Special Volunteer- Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA 2002-2002: Consultant, Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA 2001-2001: Tutor, Department of Radiation and Environmental Biology, Institute of Nuclear Physics, Krakow, Poland, financed by European Union Grant Nr. QLK4-CT-2000-00091 2001-2001: Consultant, Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA 1997-2000: Postdoctoral Fellow, Laboratory of Pharmacology and Chemistry, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA 1997-1997: Research Assistant, Department of Cancer Pathomorphology Oncology Center, Krakow, Poland 1996-1997: Research Specialist, Miraculum Cosmetic Co., Krakow, Poland 1990-1995: Postgraduate Research Student, Laboratory for Radiospectroscopy of Cancer, Jagiellonian University, Poland 1990-1990: Research Assistant, Biophysics Department, Jagiellonian University, Poland Societies: American Society for Photobiology Polish Society for Radiation Research PanAmerican Society for Pigment Cell Research Honors and Awards: \"Who is Who in the World\" 2001 edition \"Chancellors List\" 2005 Edition Original Journal Publications: 1. B.M. Kukielczak, K.A. Cieszka, Z.T. Matuszak, W.K. Subczynski, Experimental Photodynamic Therapy of Bomirski Hamster Melanoma using Merocyanine 540 and Visible Light, Current Topics of Biophysics, 1995,19(2): 66-70. 2. B. Romanowska, B. Kukielczak, J.Bryk, B. Mirkiewicz-Sieradzka, J. Heitzman, S.J. Lukiewicz, New technique for implanting Bomirski melanoma into the anterior chamber of Syrian hamster Eyes, Klinika oczna, Krakow, 97:324-327, 11- 12/1995, (in Polish with English Summary). 3. P. Bilski, B.M. Kukielczak, C.F. Chignell, Photoproduction and Direct Spectral Detection of Singlet Oxygen (•O2) in Keratinocytes, Photochemistry and Photobiology; 1998, 68(5): 675-678. 4. B. Kukielczak, B. Romanowska, J. Bryk, Gamma Radiation and MC 540 Photosensitization of Melanoma in the Hamster Eye, Melanoma Research; 1999 (9): 115-124. 5. K. Urbanska, B. Romanowska, M. Elas, S. Pajak, E. Paziewski, J. Bryk, B. Kukielczak, A. Slominski, H. Zygulska-Mach, S. Lukiewicz, Experimental Ruthenium Plaque Therapy of Amelanotic and Melanotic Melanomas Growing in the Hamster Eye, Melanoma Research, 2000,10(1): 1-10. 6. J.J. Inbaraj, B. Kukielczak, P. Bilski, S. Sandvik and C.F. Chignell, Photochemistry and Photocytotoxicity of Berberine Alkaloids from Goldenseal (Hydrastis canadensis L.) 1. Berberine, Chemical Research in Toxicology, 2001, 14 (1529- 1534); 7. J. E. Roberts, B. Kukielczak, Dan-Ning Hu, D. S. Miller, P.Bilski, R. H. Sik, A. G. Motten, C. F. Chignell, The role of A2E in protection or enhancement of light damage in human retinal pigment epithelial cells, Photochemistry and Photobiology, 75:184-190, 2002 8. Inbaraj JJ, Kukielczak BM, Chignell CF. Phloxine B phototoxicity: A mechanistic study using HaCaT keratinocytes. Photochemistry and Photobiology, 2005, 81: 81-88 9. Roberts JE, Kukielczak BM , Chignell CF, Sik RH, Hu DN, Principato MA, Simulated Microgravity Damage in Human Retinal Pigment Epithelial Cells, Mol Vis. 2006 May 30;12:633-8. 10. Inbaraj JJ, Kukielczak BM, P.Bilski, He YY, Sik RH, Chignell CF, Photochemistry and Photocytotoxicity of Berberine Alkaloids from Goldenseal (Hydrastis canadensis L.) 2. Palmatine, Hydrastine, Hydrastinine and Canadine. Chem Res Toxicol. 2006 Jun; 19(6): 739-44; 11. Chignell CF, Kukielczak BM, Sik RH, P.Bilski, He YY, Ultraviolet A sensitivity in Smith-Lemli-Opitz syndrome: Possible involvement of cholesta-5,7,9(11)-trien-3 beta-ol. Free Radic Biol Med. 2006 Jul 15;41(2):339-46. Epub 2006 Apr 30. Manuscripts in Preparation: B. Kukielczak, R.H. Sik and C.F. Chignell, Effect of Magnetite Particles on Merocyanine 540 (MC540) Phototoxicity in Human Erythrocytes and L1210 Cells. B. Kukielczak and C.F. Chignell, Comet Assay of Lomefloxacin/UVA induced DNA Damage in Human Keratinocytes. B. Kukielczak, P. Bilski, S. Sandvik and C.F. Chignell, Photochemistry and Photocytotoxicity of Fluoroquinolone Antibiotics. J.J. Inbaraj, B. Kukielczak, P. Bilski and C.F. Chignell, Photochemistry and Photocytotoxicity of the UVB sunscreen 2-phenylbenzimidazole-5-sulfonic acid. J.E. Roberts, B. Kukielczak, C. Chignell, P. Bilski, R. Sik, D.N. Hu, K. Nakanishi, The Mechanism of Visible Light Damage in Human Retinal Pigment Epithelial Cells. Abstracts Presented at Conferences: 1. B. Romanowska, J. Bryk, B., Mirkiewicz-Sieradzka, B. Kukielczak, J. Heitzman, A New Technique For Anterior Chamber Implantation of Experimental Malignant Melanoma - Florence, March, 3-5, 1994; 2. B. Romanowska, B. Mirkiewicz-Sieradzka, J. Bryk, B. Kukielczak, J. Heitzman, H. Zygulska-Mach, The influence of ionizing radiation and photodynamic treatment (PDT) on the experimental malignant melanoma in the anterior chamber of the hamster, Montpellier, October, 15/19, 1994. 3. B. Romanowska, B. Mirkiewicz-Sieradzka, J.Bryk, B. Kukielczak, J. Heitzman, H. Zygulska-Mach; Radiotherapy and photodynamic treatment (PDT) on an the experimental malignant melanoma in the anterior chamber of the hamster; Milano - Centro Congressi Milanofiori, June 25-29, 1995. 4. B.M. Kukielczak, C.F. Chignell; Coumarin-3-Carboxylate Acetoxylmethyl Ester: A Potential Fluorescent Intracellular Probe for Hydroxyl Radicals; 25th Annual Meeting of the American Society for Photobiology, St. Louis, Missouri, July 5-10, 1997. 5. B. Kukielczak, C.F. Chignell; Effect of Peroxides and UVA Irradiation on Human Keratinocytes - 26th Annual Meeting of the American Society for Photobiology; Snowbird, Utah, July 11-15, 1998; 6. P. Bilski, B.M. Kukielczak, C.F. Chignell; Photoproduction and Direct Spectral Detection of Singlet Molecular Oxygen (1O2) in Keratinocytes Stained with Rose Bengal - 26th Annual Meeting of the American Society for Photobiology; Snowbird, Utah, July 11-15, 1998; 7. C.F. Chignell, P. Bilski, B. Kukielczak, S. Sandvik; Photochemical Reactions and Photocytotoxicity of Berberine - 26th Annual Meeting of the American Society for Photobiology; Snowbird, Utah, July 11-15, 1998; 8. B. Kukielczak, R.H. Sik, C.F.Chignell, Effect of Magnetite Particles on Merocyanine 540 (MC540) Phototoxicity in Human Erythrocytes and L1210 Cells, 27th Meeting of the American Society for Photobiology; Washington, DC, July 10-15, 1999. 9. B. Kukielczak, C.F. Chignell, Comet Assay of Photoinduced DNA Damage by Fluoroquinolones, 27th Meeting of the American Society for Photobiology; Washington, DC, July 10-15, 1999. 10. B. Kukielczak, C.F. Chignell, Comet Assay of Lomefloxacin/UVA induced DNA Damage in Human Keratinocytes, 13th International Congress on Photobiology; San Francisco, CA, July 1-6, 2000. 11. J.E. Roberts, B. Kukielczak, P. Bilski, R. Sik, C.F. Chignell, D-N. Hu, The Role of A2E in the Protection and Light Damage to Human Retinal Pigment Epithelial Cells, Annual Meeting of Association for Research in Vision and Ophthalmology (ARVO), Fort Lauderdale, Florida, April 29-May 4, 2001. 12. J.E. Roberts, B.M. Kukielczak, D-N. Hu, D. Miller, P. Bilski, R.H. Sik, A. G. Motten, C.F. Chignell, Damaging Impact of Visible light and UV Radiation on Retinal Pigment Epithelial Cells, 12th Annual Meeting of Polish Society of Radiation Research, Krakow, Poland, September 10-12, 2001. 13. J.E. Roberts, B.M. Kukielczak, D-N. Hu, P. Bilski, C.F. Chignell, The Role of Lutein in Protection Against Light Damage to Human Retinal Pigment Epithelial Cells, 13th International Carotenoid Symposium, Honolulu, HI, January 6th-January 11th 2002. 14. C.F. Chignell and B.M. Kukielczak, Smith-Lemli-Opitz syndrome (SLOS) photosensitivity: Possible involvement of 7-dehydrocholesterol, 31st Annual Meeting of the American Society for Photobiology, Baltimore, MD July 5-9, 2003 15. P. Bilski, C.F. Chignell , B.M. Kukielczak, R. Sik, UVA Sensitivity in Smith-Lemli-Opitz Syndrome : Possible Involvement Cholesta-5,7,9(11)-trien-3 _-ol,, 11th Annual Meeting of the Society for Free Radical Biology and Medicine, November 17-21, 2004, St. Thomas, Virgin Islands, USA. 16. J.E. Roberts, B. Kukielczak, C.F. Chignell, B. Sik, D.N. Hu, and M. Principato, Simulated Microgravity Damage in Human Retinal Pigment Epithelial Cells, Invest. Ophthalmol. Vis. Sci. 2005 46: E-Abstract 3057 17. C.F. Chignell and B.M. Kukielczak, P. Bilski, R. Sik, UVA Sensitivity in Smith-Lemli-Opitz Syndrome: Possible Involvement of Cholesta-5,7,9(11)-trien-3 _-ol, 11th Congress of the European Society for Photobiology, Aix-les-Bains, France, September 3-8, 2005