Graduate Student Resume
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Resume:
DANIELLE N. MANDIKIAN, Ph.D.
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EDUCATION
University of California - Davis, Ph.D. Biochemistry & Molecular Biology /-/
University of California - Davis, B.S. Biochemistry & Molecular Biology -
RESEARCH EXPERIENCE
Doctoral Research, Laboratory of Dr. James Trimmer, UC Davis: / – /
The Kv. voltage gated potassium channel is widely expressed throughout the mammalian brain where it plays a major role in homeostatic regulation of intrinsic neuronal excitability. While mechanisms for how Kv. is modulated through activity-dependent changes in phosphorylation have been elucidated, little is known of molecular complexes which regulate Kv. in a cell or circuit specific manner. My dissertation work showed that in distinct regions of the mouse brain, Kv. clusters are juxtaposed to clusters of Ryanodine Receptor (RyR) intracellular calcium-release channels and a calcium dependent phosphatase previously shown to regulate Kv.. I also showed that stimulation of RyRs lead to changes in Kv. activity. Together these studies reveal that specific neurons in mouse brain have a distinct clustered localization of Kv. that provides a mechanism for coupling intracellular calcium release to phosphorylation-dependent regulation of Kv. function in a circuit specific manner.
Visiting Researcher, Laboratory of Dr. Ryuichi Shigemoto, NIPS, Okazaki, Japan: / – /
As part of an exchange program, I traveled to Japan to learn how to do electron microscopy (EM). I used EM in my dissertation to determine the ultrastructural localization of the Kv. potassium channel and its auxiliary subunit AMIGO- in multiple neuron types of the mouse brain. I prepared samples using the immuno-gold pre-embedding technique.
Undergraduate Research Assistant, Laboratory of Dr. Ken Kaplan, UC Davis: /– /
The process of cellular division occurs in distinct stages and is highly regulated through dynamic protein interactions. Passenger proteins are thought to play a role in coupling chromosome segregation with cytokinesis. In order to identify which of these passenger proteins are necessary for cytokinesis, I made mutations in passenger proteins expressed in budding yeast. I then analyzed the mutants for defects in cytokinesis using time lapse microscopy and assayed for changes in protein interactions.
Summer Intern IRTA, Laboratory of Dr. Jay Chiorini, National Institutes of Health:
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Viral based gene therapies are promising treatment options for a number of human diseases. One important challenge that must be addressed is the expression rate of the protein introduced by the viral vector. In an effort to improve expression rates for Adeno Associated Viral (AAV) constructs designed to treat Hemophilia and Sjögren's Syndrome, I used site directed mutagenesis for codon optimization. There is a redundancy in which DNA sequences (codons) will translate for specific amino acids, but the abundances of redundant codons are not equal. Mutations that swap for more abundant codons result in higher expression rates and improved the efficacy of the AAV constructs.
Summer Intern UARC, Laboratory of Dr. Jun Li, NASA AMES:
– / &
Deep brain stimulation is a surgical procedure used to treat Parkinson's Disease, but often leads to lesions along the length of the implantation. In order to minimize damage and increase sensitivity of the electrode used for stimulation, a neural interface carbon nanoelectrode biosensor was designed. I used fast scan cyclic voltammetry to look at factors to improve detection capabilities such as various dielectrics and thin films used for bio-support and small molecule filtration.
TEACHING & MENTORING
Teaching Assistant, Biochemistry Laboratory, UC Davis: / – /
Tutor, Online Chemistry and Biology Tutor, Fresno City College: -
Teaching Assistant, GRASP, Fresno City College: -
Tutor, Fresno City College: -
PUBLICATIONS
Cell type specific spatial and functional coupling between mammalian brain Kv. K+ channels and ryanodine receptors. Mandikian, Bocksteins, Parajuli, Cerda, Evans, Shigemoto and Trimmer. Submitted to Journal of Neuroscience in November and is currently in review.
Deletion of the homeostatic suppressor Kv. leads to neuronal and behavioral hyperexcitability. Speca, Ogata, Mandikian, Wiler, Eum, Wenzel, Doisy, Matt, Campi, Golub, Nerbonne, Hell, Trainor, Sack, Schwartzkroin and Trimmer. Submitted to Genes, Brain and Behavior in November and is currently in review.
A SUMO-Phospho tag team for wrestling with potassium channel gating. Mandikian D, Cerda O, Sack JT, Trimmer JS.. J Gen Physiol. May;():-.
Adeno-associated virus type (AAV): a novel AAV serotype with sialic acid- and heparan sulfate proteoglycan-independent transduction activity. Schmidt M, Voutetakis A, Afione S, Zheng C, Mandikian D, Chiorini JA..J Virol. Feb;():-.
ABSTRACTS & PRESENTATIONS
Cobb MM, Bishop H, Mandikian D, Trimmer JS. Expression and localization of Kv channels and their AMIGO auxiliary subunits. (Poster) Presented at the Annual Meeting of the Society for Neuroscience; San Diego, CA.
D. N. Mandikian, , O. Cerda, A. Evans, D. Schneider, J. S. Trimmer. Association and colocalization of plasma membrane Kv. voltage-gated potassium channels with intracellular ryanodine receptor calcium-release channels. (Poster) Presented at the Annual Meeting of the Society for Neuroscience; New Orleans,
Mandikian. The role of the HSP chaperone in coordinating anaphase events. (Talk) Presented in the and Undergraduate Research Conference, UC Davis; Davis, CA.
Mandikian, T.B. Nguyen-Vu, J. Li. Development of Dopamine Detection with Carbon Nanofiber Nanoelectrode Array. (Poster) Presented at the Summer Internship Research Conference, NASA Ames Research Center; Mountain View, CA.
RESEARCH & TRAINING GRANTS
Molecular and Cellular Biology Training Grant: -
Competitive National Institutes of Health Funded Training Grant. Grant covered all tuition and stipend.
TECHNICAL SKILLS
Acute Seizure Induction, Blue Native PAGE, Cell culture, ELISA, Fast-Scan Cyclic Voltammetry, Immunohistochemistry, Immunoprecipitation, Light Micrcoscopy, Light Spectroscopy, Monoclonal Antibody Production & Screening, Murine Animal Models, Quantitative Mass Spectrometry, Time Lapse Microscopy, Tissue Collection, Trans-cardial Perfusion, Transmission Electron Microscopy, Western Blotting, Yeast & Bacterial Genetics.
REFERENCES
James Trimmer, Ph.D. (email hidden)
Department of Neurobiology, Physiology and Behavior
University of California, One Shields Avenue, Davis CA (phone hidden)
David Speca, Ph.D. (email hidden)
Department of Neurobiology, Physiology and Behavior
University of California, One Shields Avenue, Davis CA (phone hidden)
Ken Kaplan, Ph.D. (email hidden)
Department of Molecular and cellular Biology
University of California, One Shields Avenue, Davis CA (phone hidden)
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