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santosh kumar
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M.Sc.biotechnology Fresher job
job for M.Sc.Biotechnology
SANTOSH KUMAR GOTHWAL
C/O OM Prakash Gothwal Village-Amarpura,Post-Nathawala,Via-shahpura,Dist.jaipur,Rajasthan India 303103
Contact No-09371937073; Email: gothwal_attitude@rediffmail.com
CURRENT STATUS:
Presently have been appeared in final examination for M.Sc.II, Sem. IV, Biotechnology (Results awaited)at Rajiv Gandhi Biotechnology Centre, R.T.M. Nagpur University, Nagpur INDIA under DBT SUPPORT PROGRAMME.(Ministry of science and Technology ,Govt. of India.)
ACADEMIC PROFILE:
EXAMS PASSED BOARD/UNIVERSITY SUBJECTS TAKEN PERCENT YEAR OF PASSING
M.Sc.II SEM III NAGPUR UNIVERSITY Biotechnology 76.00 JAN 2008
M.ScI(SemI&Sem II) NAGPUR UNIVERSITY Biotechnology 70.90 AUG. 2007
B.Sc UNIVERSITY OF RAJASTHAN Zoology ,Chemistry, Botany. 72.15 JUNE 2006
CLASS-XII BOARD OF SEC.EDUCATION
AJMER Physics, Chemistry, Biology & English,Hindi 73.38 APRIL 2002
CLASS-X BOARD OF SEC.EDUCATION
AJMER Maths, Science, English, Hindi, So.science,Sanskrit 73.50 JUNE 2000
ACADEMIC ACHIEVEMENTS:
Selected for M.Sc Botany in University of Delhi All India Rank-5.
Selected for M.Sc Biotechnology in R.T.M Nagpur University May-2006 through Common Entrance Exam for Biotechnology (CEEB) conducted by J.N.U, Delhi & programme supported by DBT under Ministry of Science & Technology Govt of India.
Qualified GATE-2007 with ALL INDIA RANK- 796 (Reg No. XL 230090)
Qualified for M.Sc.Chemistry in University of Rajasthan in May 2006
Qualified for M.Sc.biotechnology through IIT-JAM Conducted by IIT Guwahati in 2007.
Screened in Enteranced exam.conducred by Natioal Brain Research Center for integrated Ph.D. programme.
PROJECTS- CENTRAL DRUG RESEARCH INSTITUTE (DIV.OF TOXICOLOGY)
PROJECT TOPIC-Assesment of DNA damage on peripheral blood leucocytes and Macrophages after Lipopolysaccharide exposure in In vivo and In vitro systems.
Summary-Lipopolysaccharide (LPS) is an prototypic endotoxin and well known inflammation causing agent.It is regularly used by researchers to explore the pathogenesis of infalmatory diseases.LPS acts via bindind to several receptors like CD-14,TLR4/M2 and could cause the release of several proinflamatory cytokines could led the cells towards death via apoptosis.
Leucocytes are considered as the phagocytic cells of body and first affected in any pathogenesis.In the present study we explore the LPS mediated effect on peripheral blood leucocytes and macrophages via COMET ASSAY.Both In vitro and In vivo studies were performed for investigation.We also explore the effect of compound 2-oxo-pyridine on LPS mediated DNA damage,by COMET ASSAY,SINGLE CELL GEL ELECTROPHORESIS AND DNA FRAGMENTATION.
SEMINARS AND CONFERENCE:
Seminar presented on regulation of CELL CYCLE in Eukaryotic models at Molecular level in M.Sc. part I at R.G.B.C.RTMNU ,Nagpur
Seminar presented on Recombinant DNA Technology in M.Sc.Part II at RGBC,RTMNU,Nagpur.
Participated in “International Conference on Innovations in Biotechnology and their Applications, GLOBAL SUSTAINABLE- 2nd BIOTECH CONGRESS (2006) as volunteer and member of registration committee from 18th-21st Dec 2006.
PRACTICAL SKILLS:
Cell Culture techniques:
Media preparation for in vitro cultures ,Trypsinization of cell lines ,subculturing ,passaging, Isolation of cell clones.
Spectroscopic Techniques:
UV Spectroscopy, infrared spectroscopy, flourescence spectroscopy, calorimetry.
Molecular Biology Techniques:
Standard and real time PCR, plasmid based cloning, cloning into bacterial expression and phage vectors, RFLP, isolation of genomic DNA from bacterial, animal and plant cells, isolation of plant and yeast mRNA, isolation of plasmid DNA, restriction digestion and ligation (recombinant DNA technology), transformation (heat shock) in E.coli, southern blotting, protein isolation (SDS PAGE), purification, estimation.
Toxicological Assays:
1. Comet Assay for cell viability and cytotoxicity.
2. Hoescht assay to evaluate the apoptosis in cell lines or in vivo models.
3. DNA Fragmentation of Drug Treated cell at different concentrations.
Neurobiological Techniques:
Short term brain culture, detection of neuropeptides using bright field and flourescence microscopy, stereotaxic implantation of the cannula and intra-cerebroventricular administration in rats, application of behavioral models with specific reference to anxiety and stress.
Other Techniques: Animal house maintenance and animal care (Sprague dawley rats and Charles foester rats).
Instruments Handled:
Gel Documentation
Gel Electrophoresis.
Spectrophotometer (UV – Vis.).
Centrifuges (including cooling centrifuge)
Plant Growth Chamber/ Tissue Culture Instruments
ELISA Reader, CO2 Incubator, Thermal Cycler (PCR), Electrophoresis Units.
COMPUTER SKILLS : Windows XP, Vista and Linux (OS). Adobe Photoshop,
Corel Draw, Graph pad and Origin 50.
CAREER PLANING : To pursue research in the field of Molecular Biology ,Genetics and Pharmaceutical biotechnology
HOBBIES : Reading, Singing,Playing Cricket and Chess.
STRENGTH : Hard work, sincerity, honesty and commitment.
PERSONAL DETAILS
Date of Birth : 26th Octomber 1985
Sex : Male
Nationality : Indian
Marital Status : Single
Language known : English, Hindi, Rajasthani, Marathi.
DECLARATION
I hereby declare that the information furnished above is relevant and correct to
the best of my knowledge.
Yours faithfully
Santosh Kumar Gothwal
REFERENCES:
1. Dr. S. U. Meshram,(M.Sc. (Micro.), Ph.D. IARI, New Delhi,
Post Doc. Holland) Course Coordinator,
Rajiv Gandhi Biotechnology Centre,
DBT SUPPORT PROGRAMME,
R.T.M. Nagpur University, Nagpur.
E-Mail-rgvbc_sum123@rediffmail.com
2. Dr.Sarika Singh (Scientist ,div.of toxicology)
Central Drug Research Institute
Lucknow (India)
E-Mail –ssj3010@yahoo.co.in
Contact no.-0091+9335928432
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