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Recruiting Administrator Resume
larissa
US
10+ yrs
Yes
Yes
scientist III
project leader
Larissa V. Ponomareva 9154 Tabor Ct, Florence, KY 41042
Ponomareva@insightbb.com • Cellular 859-760-2321 • Home 859-384-6057
Summary of Qualifications
· Sixteen years scientific research experience and 1 year of teaching undergraduate and graduate courses in Biochemistry, Immunochemistry, Biomembranes, Ecology
· Experience includes 16 years biochemistry research, 14 years molecular biology research, 10 years cell biology, 10 years physiology research, 2 years electrophysiology research, and 3 years managerial and custom services expertise
· Authored 11 scientific research publications in peer reviewed journals, 4 as first author and 3 as second author
· Independent research projects include cloning of fish cytochromes P-4501A1 and 1A3 genes, studies of fish P-450 transcriptional regulation in comparison to murine P-450 promotor, development of transgenic zebra fish with GPF gene indicator and transgenic mice with degradable GFPd2 gene and period 1 and fos promotors, studies of retinal glutamate receptors nitric oxide modulation and AMPA glutamate receptors cloning and expression, studies of cell signaling of chemokine mim-1 and its role in osteoblast cell differentiation and mineralization, and effect of proteasome inhibitors on NFkB signaling and efficacy of radiation
· Managed the successful completion of over 1000 custom research assignments and projects on the contract with US EPA
¨ Managed the launch of the Research Core Facilities at the US EPA NERL; developed training and business strategies plan for Core Research Facilities on the contract with US EPA
¨ Developed new polymorphic genetic markers and indicators for environmental exposure
¨ Implemented efficiencies that improved throughput of custom services while reducing labor, materials, and standard cost of product by 25%
¨ Edited scientific research articles for laboratory colleagues
¨ Generated the transgenic mice with degradable GFPd2 and c-fos and period-1 promotors for circadian clock research project
¨ Discovered that nitric oxide and cGMP modulate retinal glutamate receptors
¨ Cloned and sequenced two ionotropic glutamate receptors and two cytochrome P-450 genes
¨ Discovered the role of osteoclast-secreted chemokine Mim-1 in osteobasts differentiation and bone matrix mineralization
¨ Discovered that proteasome inhibition could enhanced radiation sensitivity in prostate cancer cells but does not have this effect in normal cells and this could be a potential way to improve efficacy of radiation in prostate cancer patients
Professional Experience
Studying the molecular mechanisms of pain regulation in pancreatic cancer and arthritis animal models
Directed 5 - 6 people in the daily process of custom sequencing and genotyping for the US EPA researchers and ensured effective follow-through on all assigned tasks as well as strategic delegation of tasks to qualified individuals. The group ran from 200 to 1000 sequencing or genotyping samples per day, my responsibilities included troubleshooting, customer interface, data analysis and data presentation. One of my main contributions was process improvement and experimental design of custom research projects, developing Work Plans, SOPs and quality assurance reports.
Acquired knowledge in biological effects of proteasome inhibitor MG-132 on NFkB regulation in prostate cancer animal model.
Research Associate (Department of Internal Medicine)
Studied cell signaling of chemokine mim-1 and its role in osteoblast cell differentiation and bone mineralization
Postdoctoral Research Fellow/Research Associate (Department of Physiology)
Carried out research on function, modulation and expresiion of ionotropic glutamate receptors from fish retina, directed project on circadian clock study, generated transgenic GFP mice.
Postdoctoral Research Fellow (Department of Environmental Health)
Cloned, sequenced, and studied transcriptional regulation for trout cyt. P-4501A3 and 1A1 genes and participated in developing transgenic GFP-zebrafish-biomonitor (environmental “sentinel” for aquatic pollution)
Assistant Professor (Department of Physico-Chemical Biology)
Taught biochemistry, immunochemistry, ecology and biomembranes courses for undergraduate and graduate students
Senior Scientist (Institute of Biology)
Conducted research on effect of environmental pollution on induction of cytochrom P-450 isoforms from freshwater fish species including endemics from Lake Baikal
Ph.D. student (Department of Biophysics)
Irkutsk State University, Irkutsk, USSR 08/1985 to 09/1986
Research Fellow (Department of Physico-Chemical Biology)
Education
Moscow State University, Moscow, Russia 1990
Irkutsk State University, Irkutsk, USSR 1985
Skills
• Teaching: Biochemistry, immunochemistry, biomembranes, ecology
• Management: Responsible for production of high-throughput contract sequencing and genotyping core facility. Duties included managing high-throughput robotic DNA and RNA extraction/purification, DNA genotyping and sequencing, non-isotopic library screening, large-scale gene-specific PCR amplification for genotyping and polymorphism studies, assay development and sequence data analysis, RT-QPCR analysis, preparation of Lab Work Plans, Quality Management Plans, SOPs, monthly QA/QC reports, review of all data and staff reports, managing the laboratory supply budget and supervision of a staff of scientists (PhD, MS & graduate students) and technicians (BS).
• Molecular biology expertise in: Gene Chip Microarrays, cDNA and genomic libraries construction and screening, DNA cloning, DNA extraction: minipreps, maxipreps (manual and BioRobot workstations), CsCl DNA purification; Southern, primers design, site-directed mutagenesis, footprinting, manual and high through-put automatic sequencing, SSCP, SNP, genotyping (microsatellite), Genome Walk, RNA purification, Northern, RNase mapping, in vitro transcription, RT-PCR, single cells RT-PCR, in situ hybridization, RACE, primer extension, RT-QPCR (TaqMan, SYBR Green), differential display
• Biochemistry expertise in: EMSA, SDS PAGE, 2D gels, Western Blotting, HLPC, ELISA, IHC, protein extraction, dialyze and protein purification, immunoprecipitation, binding assays, receptor/ligand interaction, protein expression in bacterial and mammalian cell lines, labeling/conjunction, immunocytochemistry, enzyme assays and enzyme kinetics, IEC, TLC, mass-spectrometry, GLC, flow cytometry and cell sorting (FACS), spectrophotometry, spectrofluorimetry
• Computers and programming: Word processing, spreadsheet, PowerPoint and computer data analysis (BLAST, SigmaPlot, Excel, Axon Workbench, Clampex5-8, AtlasImage2.0, etc); large-scale genomic analysis
• Tissue culture expertise in: Transient transfections of HEK 293 and N2A cell lines (calcium-phosphate method, Lipofectamin, Fugen, gene gun, electroporation), stable retroviral (Clontech) cell transfection, immunocytochemistry, neuronal and hepatocyte primary culture, growth of retinal cells, fibroblasts, culture MG-63, human stromal cells, C2C12, cancer cell lines, clonogenic survival assay (+/-presence of drug, +/- radiation)
• Electrophysiology expertise in: Whole cell patch-clamp recording from retinal horizontal cells, HEK 293 cells, recording data acquisition analysis with Clampex5
• Imaging: EGFP imaging analysis, fluorescent calcium imaging analysis with fura-2
• Microscopy: light-inverted microscope, epifluorescence, confocal, electron-microscopy
American Association for the Advancement of Science
Association for Research in Vision and Ophthalmology
Society for Neuroscience
Russian Biochemical Society
2004 Managers School Training and Negotiation Skills Training completed at Xavier Consulting Group, Xavier University, Cincinnati,OH USA.
1991 International Biotechnology Center; Biomonitoring, Environmental Health and Ecology Training Program, Moscow State University, Completed with Certification of Advanced Training
1996 Fight for Sight Research Fellowship Award, Prevent Blindness America, ARVO, USA
1995 Award for best poster presentation at Society of Neuroscience Conference, University of Kentucky, Lexington, KY, USA
1991 COMECON Fellowship, Biotechnology and Ecology Training Program, Moscow
1986-1990 Moscow State University Fellowship, Moscow
1985 Award of Excellence for M.S.D. Dissertation Research, USSR Competition of Graduate Student Dissertation Research, Moscow
1981-1985 Lenin Fellowships, Irkutsk State University, Irkutsk
1981 Novosibirsk State University Fellowship, Novosibirsk
1980 Gold Medal for graduation with honors, High School, Irkutsk
Furnished upon request
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